- Associate Professor, Biological Sciences
- PhD, Umea Univeristy, Sweden, 2005
Dr. Werner became interested in the question “What is life?” as a four-year old child in his parent’s garden in former East Germany. With the age of 10, he began to develop a life-long interest in the biology of butterflies and moths. He has been breeding and collecting them ever since. For his Master’s thesis, he decided to shift his focus to molecular biology because this was a newly emerging field of biology that promised new jobs.
Thus, Dr. Thomas Werner studied the human heart disease-causing virus Coxsackie B3 at the molecular level at Friedrich-Schiller-University Jena in Germany. After the fall of the Berlin Wall, Dr. Werner made one of his childhood dreams become true and moved to Sweden. He spent seven years in Umeå, working on his Ph.D. thesis about the innate immune response in the fruit fly Drosophila melanogaster.
During this time at Umeå University in Dr. Dan Hultmark’s lab, he discovered and described a new family of immune genes that we humans share with flies and many other animals. In 2005, Dr. Werner shifted his research focus towards evolution of development (evo-devo) and worked as a postdoctoral fellow at the University of Wisconsin-Madison in Dr. Sean B. Carroll’s lab, where he established the fruit fly Drosophila guttifera as a new transgenic model organism to investigate how complex animal color patterns evolve.
Left wing: natural color. Right wing: Vein Spot Enhancer (turquoise) and Intervene Shade Enhancer (red) drive the yellow gene to make the color pattern seen on the left.
Current Research Project 1: The evolution and development of complex animal color patterns in Drosophila
Animal color patterns such as zebra stripes, leopard spots, and the myriad variants of butterfly wing color patterns are known to play important ecological and physiological roles in the life of animals and are crucial for the survival of species. Scientists first tried to solve the secret of animal color patterns with mathematical approaches to find models that could explain how these patterns developed. In 1952, Turing, the inventor of the computer, proposed the famous reaction-diffusion model in which a short-range acting activator molecule diffuses from a source to stimulate color production, while a long-range acting inhibitor molecule prevents pigmentation. Using the spectacularly ornamented fruit fly Drosophila guttifera, we identified that the Wingless morphogen had evolved a new function in this species by activating the yellow gene, causing many black melanin spots to appear on the wings. Humans also have the wingless gene, which is a known proto-oncogene (called Wnt-1). Wnt-1 is overexpressed in many tumors, which causes the tumors to proliferate. We are currently investigating if the color patterns on the abdomen and thorax of Drosophila guttifera are generated by the same pathway as on the wing, or if other proto-oncogenes have evolved a link to pigmentation. Elucidating the pigmentation pathways in this fruit fly can help us understand how cancer-inducing pathways are regulated and how they have evolved.
Current Research Project 2: The evolution of mushroom poison (alpha-amanitin) resistance in Drosophila
Just one Death Cap or Destroying Angel mushroom contains enough toxin to kill a person. Alpha-amanitin is the principal toxin in these deadly-poisonous mushrooms. However, poisonous mushrooms generally contain a mixture of different toxins that target various tissues. Because alpha-amanitin targets the RNA-polymerase II and renders it non-functional, almost all eukaryotic organisms are very sensitive to this toxin. Only a handful of organisms on the planet can eat these mushrooms and survive without harm. Some Drosophila species belong to the resistant organisms. Interestingly, their RNA-polymerase II is highly sensitive to the toxin. Thus, there must be detoxification and/or excretion mechanisms that allow these flies to consume these mushrooms, so that the toxins don’t get to their targets. Being able to eat poisonous mushrooms both provides a unique niche in the eco-system and also kills parasites that would otherwise render the flies sterile. The main goal of Dr. Werner’s research is to elucidate the molecular-genetic mechanisms that cause alpha-amanitin resistance in Drosophila.
Drosophilids of the Midwest and Northeast
by Thomas Werner, Tessa Steenwinkel, and John Jaenike
Links of Interest
- Developmental Biology
- Werner, T., Steenwinkel, T., Jaenike, J. (2017) Drosophilids of the Midwest and Northeast. (Version 2) J. Robert Van Pelt and John and Ruanne Opie Library, Michigan Technological University. Houghton, Michigan. ISBN: 978-1-7326524-0-8 (E-Book, 345 pages) Read More
- Scott Chialvo C. H. and Werner, T. (2018) Drosophila, destroying angels, and deathcaps! Oh my! A review of mycotoxin tolerance in the genus Drosophila. Frontiers in Biology. April 24, 1-12. Read More
- Werner, T. (2017) The Drosophilids of a Pristine Old-Growth Northern Hardwood Forest. The Great Lakes Entomologist, 50 (2), 68-78. (Cover) Read More
- Werner, T. and Jaenike, J. (2017) Drosophilids of the Midwest and Northeast. River Campus Libraries, University of Rochester. Rochester, New York.
- Mitchell, C.M., Latuszek, C.E., Vogel, K.R., Greenlund, I.M., Hobmeier, R.E., Ingram, O.K., Dufek, S.R., Pecore, J.L., Nip, F.R., Johnson, Z.J., Ji, X., Wei, H., Gailing, O., Werner, T. (2017) Alpha-Amanitin Resistance in Drosophila melanogaster: A Genome-Wide Association Approach. PLoS ONE, Feb 27;12(2):e0173162. doi: 10.1371/journal.pone.0173162. Read More
- Zhang, J., Li, C., Dutta, C., Zhang, S., Tiwari, A, Werner, T., Luoc, F-T, Liu, H. (2017) A Novel Near-infrared Fluorescent Probe for Sensitive Detection of β-Galactosidase. Analytica Chimica Acta, 968, 97-104. Read More
- Koshikawa, S., Giorgianni, M.W., Vaccaro, K., Yoder, J.H., Werner, T., Carroll, S.B. (2015) The gain of cis-regulatory activities underlies novel domains of wingless gene expression in Drosophila. Proc Natl Acad Sci USA Published ahead of print doi: 10.1073/pnas.1509022112 Read More
- Mitchell, C.M., Yeager, R.D., Johnson, Z.J., D’Annunzio, S.E., Vogel, K.R., Werner, T. (2015) Long-term resistance of Drosophila melanogaster to the mushroom toxin alpha-amanitin. PLoS ONE May 15 DOI:10.1371/journal.pone.0127569 Read More
- Werner T. (2015) Leopard spots and zebra stripes on fruit fly wings. Nature Education 8(2):3 Read More
- Mitchell, C.M., Saul, M.C., Lei, L., Wei, H., Werner, T. (2014) The Mechanisms Underlying Alpha-Amanitin Resistance in Drosophila melanogaster: A Microarray Analysis. PLoS ONE. Apr 2;9(4):e93489. doi: 10.1371/journal.pone.0093489 Read More
- Bray, M.J., Werner, T., Dyer, K.A. (2014) Two genomic regions together cause dark abdominal pigmentation in Drosophila tenebrosa. Heredity 112, 454-462. (11 December 2013) doi:10.1038/hdy.2013.124 Read More
- Werner T., Koshikawa S., Williams T.M., Carroll S.B. (2010) Generation of a novel wing color pattern by the Wingless morphogen. Nature, Vol. 464, pp. 1143-1148. Read More
- Rebeiz M., Ramos-Womack M., Jeong S., Andolfatto P., Werner T., True J., Stern D.L., Carroll S.B. (2009) Evolution of the tan Locus Contributed to Pigment Loss in Drosophila santomea: A Response to Matute et al. Cell, Vol. 139, pp. 1189-1196. Read More
- Williams T.M. , Selegue J.E., Werner T., Gompel N., Kopp A., and Carroll S. B. (2008) The Regulation and Evolution of a Genetic Switch Controlling Sexually Dimorphic Traits in Drosophila. Cell, Vol. 134, pp. 610–623. Read More
- Jeong S., Rebeiz M., Andolfatto P., Werner T., True J., Carroll S.B. (2008) The Evolution of Gene Regulation Underlies a Morphological Difference between Two Drosophila Sister Species. Cell, Vol. 132, pp. 783-93. Read More
- Werner T., Borge-Renberg K., Mellroth P., Steiner H. and Hultmark D. (2003) Functional diversity of the Drosophila PGRP-LC gene cluster in the response to LPS and peptidoglycan. J Biol Chem, Vol. 278, Issue 29, pp. 26319-22. Read More
- Choe K.M., Werner T., Stoven S., Hultmark D, Anderson K.V. (2002). Requirement for a Peptidoglycan Recognition Protein (PGRP) in Relish Activation and Antibacterial Immune Responses in Drosophila. Science, Vol. 296, Issue 5566, pp. 359-62. Read More
- Werner, T., Liu, G., Kang, D., Ekengren, S., Steiner, H. and Hultmark, D. (2000). A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster. Proc Natl Acad Sci USA, Vol. 97, Issue 25, pp. 13772-7. Read More
Grants and Awards
- Huron Mountain Wildlife Foundation Grant (2018-2023)
- NIH R15 Grant (2016 - 2019)
- Huron Mountain Wildlife Foundation Grant (2014-2016)
- Distinguished Teaching Award (2013)
- The evolution and development of complex animal color patterns in Drosophila
- The evolution of mushroom poison (alpha-amanitin) resistance in Drosophila
- Introduction to Genomics (lecture) since 2016
- Genetics (lecture) since 2012
- Genetic Techniques (lab) since 2011
- General Immunology (lecture) since 2011