Paul D. Goetsch
- Dow 502
- Assistant Professor, Biological Sciences
In multicellular organisms, how do multiple cell types originate from one cell and one genome? A critical early cell fate decision is whether to develop as germline (reproductive) or soma (non-reproductive). My research focuses on assessing how the highly conserved DREAM transcriptional repressor complex maintains the germ vs. soma cell fate decision during embryogenesis. After a flurry of cell divisions from the starting “germ cell” (i.e. united egg and sperm), somatic cells begin to exit the cell cycle as the DREAM complex suppresses the proliferative germ cell program. This process is commonly reverted in cancer cells (i.e. a soma-to-germline transition) and is intentionally reversed when mammalian somatic cells are reprogrammed into induced Pluripotent Stem Cells. DREAM’s involvement in protecting somatic cell differentiation suggests that transcriptional suppression of both “germness” and the cell cycle governs the establishment of cellular identity during development. A major challenge is identifying the transcription factors whose activities are unlocked following loss of DREAM function. To address this challenge, I have developed a functional genomics pipeline that integrates new CRISPR/Cas9 genome editing tools, high-throughput sequencing analysis, genetics, biochemistry, and cell biology in the Caenorhabditis elegans model system. Using this pipeline, my ongoing projects aim to elucidate how the DREAM complex forms and functions on chromatin, what transcription factors drive the soma-to-germline transition when DREAM function is lost, and how cellular quiescence is established. We will also apply our discoveries in C. elegans to help address how soma-to-germline transitions occur in cancer cells. Each project lays the groundwork for future work addressing how transcriptional events during development establish cellular identity.
Areas of Expertise
- Molecular Genetics and Biochemistry
- Genomics / Bioinformatics
Links of Interest
- Developmental Biology
- Transcriptional Biology
- Cancer Biology
- Goetsch, Paul; University of Minnesota Duluth. Department of Biology. (2021). DREAM interrupted: Establishing a CRISPR/Cas9 functional genomics pipeline in Caenorhabditis elegans (2021-02-19). Retrieved from the University of Minnesota Digital Conservancy. Read more
- Paul J. Vorster, Paul Goetsch, Tilini U. Wijeratne, Keelan Z. Guiley, Laura Andrejka, Sarvind Tripathi, Braden J. Larson, Seth M. Rubin, Susan Strome, Joseph S. Lipsick. 2020. A long lost key opens an ancient lock: Drosophila Myb causes a synthetic multivulval phenotype in nematodes. Biol Open; 9 (5). Read more
- Vorster, P. J., Goetsch, P., & Wijeratne, T. U. 2020. A long lost key opens an ancient lock: Drosophila Myb causes a synthetic multivulval. Biology open, 9 (5).
- Paul D Goetsch, Susan Strome. 2019. DREAM Interrupted: Severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization, Genetics, Volume 221, Issue 3. Read more
- Goetsch, P. D., Garrigues, J. M., & Strome, S. 2017. Loss of the Caenorhabditis elegans pocket protein Lin-35 reveals MuvB's innate function as the repressor of Dream Target Genes. PLOS Genetics. Read more
- Marceau, A., Felthousen, J., Goetsch, P. et al. 2016. Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters. Nat Commun 7, 12301. Read more
- Mark B. Meyer, Paul D. Goetsch, J. Wesley Pike. 2012. VDR/RXR and TCF4/β-Catenin Cistromes in Colonic Cells of Colorectal Tumor Origin: Impact on c-FOS and c-MYC Gene Expression, Molecular Endocrinology, Volume 26, Issue 1, Pages 37–51. Read more
- Pike, J. W., Meyer, M. B., Goetsch, P. D., & Lee, S. M. 2011. NEW PARADIGMS FOR GENE REGULATION BY 1, 25-DIHYDROXYVITAMIN D-3. In ANTICANCER RESEARCH (Vol. 31, No. 4, pp. 1491-1491). EDITORIAL OFFICE 1ST KM KAPANDRITIOU-KALAMOU RD KAPANDRITI, PO BOX 22, ATHENS 19014, GREECE: INT INST ANTICANCER RESEARCH.
- J. Wesley Pike, Mark B. Meyer, Melissa L. Martowicz, Kathleen A. Bishop, Seong Min Lee, Robert D. Nerenz, Paul D. Goetsch. 2010. Emerging regulatory paradigms for control of gene expression by 1,25-dihydroxyvitamin D3, The Journal of Steroid Biochemistry and Molecular Biology, Volume 121, Issues 1–2, Pages 130-135. Read more
- Mark B. Meyer, Paul D. Goetsch, J. Wesley Pike. 2010. Genome-wide analysis of the VDR/RXR cistrome in osteoblast cells provides new mechanistic insight into the actions of the vitamin D hormone, The Journal of Steroid Biochemistry and Molecular Biology, Volume 121, Issues 1–2, Pages 136-141. Read more
- Mark B. Meyer, Paul D. Goetsch, J. Wesley Pike. 2010. A Downstream Intergenic Cluster of Regulatory Enhancers Contributes to the Induction of CYP24A1 Expression by 1α,25-Dihydroxyvitamin D3*, Journal of Biological Chemistry, Volume 285, Issue 20, Pages 15599-15610. Read more
- Meyer, M. B., P. D. Goetsch, and J. W. Pike. 2010. "Genome-wide analysis of the VDR." RXR cistrome in osteoblast.
- Junfa Zhu, Paul Goetsch, Nancy Ruzycki, and Charles T. Campbell. 2007. Journal of the American Chemical Society 129 (20), 6432-6441 DOI: 10.1021/ja067437c. Read more
- Zhu, J., Goetsch, P., Ruzycki, N., & Campbell, C. T. (2006). COLL 553-Heat of adsorption and structure of calcium on poly (methyl methacrylate)(PMMA) surfaces. In ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY (Vol. 232). 1155 16TH ST, NW, WASHINGTON, DC 20036 USA: AMER CHEMICAL SOC.
- S.F. Diaz, J.F. Zhu, J.J.W. Harris, P. Goetsch, L.R. Merte, Charles T. Campbell. 2005. Heats of adsorption of Pb on pristine and electron-irradiated poly(methyl methacrylate) by microcalorimetry, Surface Science, Volume 598, Issues 1–3, 2005, Pages 22-34. Read more