Michigan Tech News

Study of Key Biomolecule Earns Tolou Shokuhfar CAREER Award

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By Marcia Goodrich


Last Modified 1:53 PM, July 1, 2014

The white dots in this electron microscope image are the protein ferritin, trapped in a bubble of liquid in a graphene capsule. Tolou Shokuhfar will use this new technology to study ferritin's inner workings, with the aim of advancing cures for disease.

July 1, 2014—

Tolou Shokuhfar will be investigating the inner workings of a protein that plays a key role in human health with funding from a five-year, $400,000 Faculty Early Career Development (CAREER) Award.

Shokuhfar, an assistant professor of mechanical engineering-engineering mechanics at Michigan Technological University, will study the biomolecule ferritin, which stores iron in the body in a non-toxic, mineralized form and releases it safely. In humans, ferritin serves as a buffer between iron deficiency and iron overload, and when it malfunctions, it may be involved in a number of degenerative diseases, such as Alzheimer’s and Parkinson’s.

“If ferritin isn’t functioning well, free iron can float around in the body oxidizing tissues,” Shokuhfar said. That can cause disorders ranging from heart disease to cancer.

Shokuhfar will observe ferritin molecules using a revolutionary technique she developed with colleagues at the University of Illinois-Chicago. By encapsulating ferritin in a microscopic graphene bubble, they were able to observe the fully hydrated molecule using an electron microscope. Usually samples must be freeze-dried and sliced, because electron microscope samples are imaged in a vacuum, but this new technique allows scientists to capture images of biomolecules in their natural state.

“People have been studying ferritin for 80 to 100 years, but no one’s been able to observe its working mechanism at the atomistic level before,” Shokuhfar said. “Now we can.”

Shokuhfar will place dysfunctional and healthy ferritin in solution and view in real time what happens when iron ions are introduced to the mix. “Understanding the molecular basis of this disease can give us insights into the role of iron and help design therapeutic approaches,” she said. “I’m really looking forward to getting this project going, because I believe this research can have a big impact on human health.”

Michigan Technological University (www.mtu.edu) is a leading public research university developing new technologies and preparing students to create the future for a prosperous and sustainable world. Michigan Tech offers more than 120 undergraduate and graduate degree programs in engineering; forest resources; computing; technology; business; economics; natural, physical and environmental sciences; arts; humanities; and social sciences.