- M & M building 402
Assistant Professor, Biomedical Engineering
Zhao's lab seeks to address the challenges associated with engineering completely biological cardiovascular tissues for regenerative medicine applications. We are developing innovative cell therapies using stem cell sheets to create 3D scaffold-free tissue constructs with the structural similarity, necessary mechanical characteristics, and sufficient immuno-compatibility to facilitate functional in vivo integration. We are also trying to recreate biomimetic microenvironment for 3D tissue development by precisely manipulating biomaterials, oxygen tension, and hydrodynamic culture conditions. The underlying mechanisms that regulate the tissue development are under ongoing investigation by the experimental replication of in vivo cell-cell and cell-environment interactions.
- Cardiovascular tissue regeneration
- Stem cell therapies
- Cell sheet engineering
- 3D scaffold-free tissue fabrication
- Zhao F, Veldhuis JJ, Duan Y, Yang Y, Christoforou N, Ma T, Leong KW. Low oxygen tension and synthetic nanogratings improve the uniformity and stemness of human mesenchymal stem cell layer. Molecular Therapy, 2010;18:1010–1018.
- Zhao F and Ma T. Low-oxygen pretreatment enhances endothelial cell growth and retention under shear. Tissue Engineering, 2009; 15: 135-146.
- Zhao F, Grayson WL, Ma T. Perfusion affects the tissue developmental patterns of human mesenchymal stem cells in 3D Scaffolds. Journal of cellular physiology, 2009; 219: 421-429.
- Zhao F, Chella R and Ma T. Effects of shear stress on 3-D human mesenchymal stem cell construct development in a versatile perfusion bioreactor system: experiments and hydrodynamic modeling. Biotechnology and Bioengineering, 2007; 96: 584-595.
- Zhao F, Grayson WL, Ma T, Bunnell B, and Lu WW. Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development. Biomaterials, 2006;27: 1859-1867.