Standard Operating Procedure—Blood

Background/General Information

Exposure to blood and other body fluids is a potential hazard since they may contain bloodborne pathogens that cause diseases such as hepatitis and AIDS. It is reasonable to expect that custodial staff may be exposed to blood and bloodborne pathogens as a part of their normal duties. For example, they may be required to clean floors, plumbing fixtures, and other items that are soiled with blood or other body fluids. Employees can significantly reduce their chances of being infected by bloodborne pathogens by taking appropriate precautions. Wearing protective gloves can prevent infection since pathogens may enter the body through cuts, sores, rashes, or other injuries to the skin. Similarly, wearing safety glasses and a face covering can prevent infection by protecting your eyes, nose, and mouth.

Although emphasis is placed on preventing hepatitis and AIDS, there are many other diseases caused by bloodborne pathogens. Following the work practices and using the personal protective equipment (PPE) described below will minimize the chances that you may become infected by any of these bloodborne pathogens. If any situation arises where you believe that following these procedures presents a risk to your personal safety, you should contact your supervisor before proceeding.

Hazards / Risks


Hepatitis is a disease affecting the liver. Although there are many causes of hepatitis, the most common cause in the United States is infection by the hepatitis A, B, or C virus. Because hepatitis B and C viruses are also bloodborne pathogens, they are a particular concern for anyone who may be exposed to blood or other body fluids as part of their work responsibilities. The symptoms of hepatitis may range from mild or unnoticed to severe, depending on the patient and on the virus that causes the infection. Severe symptoms may include fever, fatigue, nausea, vomiting, and joint pain, and may last from a few weeks to several months. In some cases patients do not make a full recovery. Although the symptoms may go away, the virus may persist as a chronic infection. Chronic infection may lead to liver disease, cirrhosis, cancer, and possibly early death.

Hepatitis B Virus (HBV)

The most serious occupational exposure is an injury caused by a needle or other sharp object that is contaminated with HBV. If untreated, the chance of becoming infected by the virus after this type of exposure is about one in three. Hepatitis symptoms may develop in 30–50 percent of people who become infected. Of those infected, 95 percent usually recover completely. The remaining 5 percent may become chronically infected. The majority of chronically infected individuals have no symptoms of infection until the onset of cirrhosis or liver disease. Chronic infections may be treated but not cured with antiviral drugs. The disease can be prevented by receiving the HBV vaccination.

Hepatitis C Virus (HCV)

The chance of becoming infected by HCV after an occupational exposure to blood from a person infected with HCV is about 1.8 percent. The symptoms of HCV infection are similar to those caused by HBV, but the symptoms only occur in only 20–30 percent of infected individuals. Chronic infection that may lead to liver disease or liver cancer is expected to occur in 75–85 percent of infected individuals. There is no HCV vaccine, but the disease may be treated with antiviral drugs.

Human Immunodeficiency Virus (HIV) and AIDS

The overall risk of contracting HIV as a result of an occupational exposure to blood or other potentially infectious materials is low (estimated at 0.3 percent). Within a few weeks of being infected with HIV, some people may develop flu-like symptoms that last for a week or two, while others may have no symptoms at all. AIDS occurs in the late stages of HIV infection, when a person's immune system is severely damaged and has difficulty fighting diseases. Untreated HIV infection is also associated with cardiovascular disease, kidney disease, liver disease, and cancer. There is currently no HIV vaccine or cure for AIDS. However, there are treatments that can help people living with HIV have full and productive lives.

Occupational Exposures

If you are exposed to blood or other potentially infectious materials, follow these guidelines:

  • Skin exposure: Vigorously wash affected skin with plenty of soap and water for up to 10 minutes. If necessary, remove contaminated clothing. Avoid the use of bleach or other disinfecting agents that are caustic to the skin.
  • Cuts or punctures of the skin: Wash the affected area thoroughly with soap and water, and rinse the area for up to 10 minutes. Allow small cuts and punctures to bleed freely. The application of antiseptics or disinfectants may be beneficial; however, avoid the use of bleach and other disinfecting chemicals that are caustic to the skin.
  • Eye exposure: Wash eyes for at least 10 minutes with copious amounts of water, lifting the upper and lower eyelids occasionally. If only one eye is affected, be careful not to wash the contaminant into the unaffected eye.
  • Report the incident to your supervisor and seek follow-up medical attention and evaluation.

Personal Protective Equipment (PPE)

Protect yourself from exposure.


  • Gloves made from nitrile or another appropriate material must be worn when cleaning and disinfecting areas contaminated by blood or other potentially infectious materials.
  • Never touch your eyes, nose, mouth, or face while wearing gloves.
  • To prevent the spread of contamination, gloves must be removed before touching “clean” surfaces such as doorknobs, cell phones, etc.
  • Do not wash or reuse disposable gloves.
  • Gloves must be changed when they become contaminated, or if they are torn, punctured, or otherwise compromised.
  • Gloves must be removed in a manner that prevents the unintentional transfer of hazardous or infectious material from the outside contaminated surfaces of the glove to unprotected skin or clothing.
  • Consider wearing two pair of gloves for particularly hazardous work, as this permits removal of a contaminated outer glove with minimal risk of exposure.
  • Contaminated single-use gloves are disposed as biological waste.

Eye and Face Protection

  • Wear safety glasses and a face mask or face shield as necessary to protect your eyes and face from splashes or spray. 
  • Do not put on or remove face/eye protection while wearing gloves that are potentially contaminated.
  • Individuals who wear contact lenses should also wear eye protection. Contact lenses do not provide protection to the eyes. Foreign material splashed into the eye may become trapped under the contact lens and result in more serious injury.

Additional PPE

Coveralls, aprons, shoe covers, etc., may be necessary for cleanup of large amounts of blood or other body fluids. 

Cleanup Procedure

Materials Needed

  • Oxivir TB or another approved disinfectant, such as a bleach solution
  • PPE (gloves, coveralls, shoe covers, safety glasses, face mask)
  • plastic bags/liners
  • five-gallon bucket with lid, labeled for biohazard waste
  • brush and dustpan, or tongs or forceps for picking up sharps
  • disposable paper toweling


Evaluate the area that needs cleaning and select appropriate PPE.

  • For small amounts of blood or other potentially infectious materials, a pair of gloves may be sufficient to protect you from exposure.
  • For larger amounts of blood or body fluids, coveralls, shoe covers, safety glasses, a face mask, and a pair of gloves may be required to protect you from exposure.
  • Protecting yourself should be your priority. Wearing more PPE than necessary is better than not wearing enough. If you are unsure about selecting appropriate PPE, talk to you supervisor.

Decontamination and Cleaning

  1. Oxivir TB is recommended for decontamination and cleaning. If you are planning to use bleach as an alternative, prepare a fresh bleach solution by adding one cup of bleach to one gallon of water. Do not use bleach on carpeted areas or fabric that is not color-fast (always test in an area that is not easily seen before proceeding).
  2. Cover the area with paper towels to avoid tracking the blood or potentially infectious materials to other areas.
  3. Pick up any sharp objects (broken glass, pieces of metal, needles, etc.) using tongs, forceps, or a dustpan and brush, and place them into a five-gallon bucket that is lined with a plastic bag/liner.
  4. Spray or pour Oxivir TB onto the paper towels until they are saturated. If you are using bleach, pour the freshly mixed bleach solution over the paper towels until they are saturated.
  5. Make sure that the surface remains wet for at least one minute when using Oxivir TB. Up to 10 minutes may be required for certain pathogens (see Oxivir TB label). Always wait at least 10 minutes when disinfecting with bleach.
  6. Working from the edges toward the center, carefully clean the area using additional paper towels as needed to absorb the disinfectant.
  7. If a scrub brush is needed to remove the material from hard porous surfaces such as concrete, be sure you are wearing appropriate PPE to protect yourself from splashes or spray.
  8. If cleaning large amounts blood, a second application of disinfectant may be necessary to ensure that surfaces are adequately cleaned and disinfected.
  9. After the contaminated area has been cleaned, use fresh water to remove bleach/disinfectant residue from all surfaces. 


  1. Place disinfected material (broken glass, paper towels, gloves, and other disposable materials) into a five-gallon plastic bucket that is lined with a plastic bag/liner. Use a double bag if necessary to prevent leakage.
  2. Carefully gather the bag around waste and tie the top. Try not to trap excess air the bag when tying the top.
  3. Secure the lid on the five-gallon bucket.
  4. Contact the biosafety officer for a pickup and disposal ( or 906-487-2131).
  5. Keep the biohazard waste in a secured area until it can be picked up.

Decontaminate Reusable Equipment

Use Oxivir TB to decontaminate all reusable tools or protective equipment used in the cleanup. This includes dustpans, brooms, forceps, buckets, mops, etc. Anything that cannot be effectively decontaminated (the disinfectant must be able to make contact with all surfaces) must be disposed as waste.

Wash Your Hands

If handwashing facilities are not available at the job site, use disinfectant wipes and then wash your hands as soon as possible.